Abstract:
Medical Imagery, the most popular and challenging imagery techniques which
have been a point of interest for many studies nowadays. Light microscopy requires
proper illumination to acquire gigapixel high resolution and wide field of view images
at the same time. Whereas incandescent light was once the only source of illumination,
nowadays specialized light sources, such as laser emitting diodes (LED), lasers or arc
lamps, are now being used. Light-emitting diodes (LEDs) have evolved into a serious
option for practically all types of illumination in light microscopy due to their great
efficiency and brightness. They are long-lasting, require no expensive electronics, and
can be switched in nanoseconds. Aside from that, they have a narrow bandwidth and
are available across the UV/Vis/NIR spectrum. As a result, they seem to be the most
suitable illumination for different microscopy techniques. With a color temperature
spanning from 2,600 to 5,000 K, the white LED is best choice for bright-field
illumination, with the added benefit of being energy efficient.
On the other hand, lasers produce dense packets of monochromatic light that
are extremely collimated and coherent, resulting in a tight beam with a very low rate
of expansion. The extremely pure wavelength ranges emitted by the laser have a
bandwidth and phase relationship that is unequalled by tungsten-halogen or arc-
discharge lamps when compared to other light sources. As a result, laser light beams
can travel long distances and be expanded to span apertures or concentrated to a very
small, bright spot, which reduces diffraction and maintain the light intensity. In this work we will explain in detail the differences between them using blind image quality
assessments.
